Slavkovský Rastislav Ph.D.
Postdoctoral training
About me
I am a research scientist passionate for molecular and cellular biology, masive parallel sequencing and gene-expresion analysis. I always enjoy datamining of biologicaly relevant data and the comparison of data with already known information. I was involved in different studies such as wound healing characterization, experimental diabetes mellitus, effect of potentially active compounds or radiation on cells in vitro. I was responsible for a developement of a small laboratory for studing microbial biofilm in vitro. With my collegues, we are working on the developement of NGS methods for predicive diagnostics of cancer and methylome profiling. Recently we successfully released fastGEN NGS technology products together with our industrial partners. Currently we are also studying how the somatic mutations in blood cells that are appearing during aging and are connected to different vascular pathologic processeses.
Journals
Master mentorship
Podhorská Natália
Epigenetic changes detection in cancer cells
Csergeova Lucia
Genotyping of tumor somatic biomarkers using multi-parallel sequencing technologies with molecular barcodes features
Vysloužilová Lenka
Significance assessment of gene variants identified by next-generation sequencing associated with inherited cardiomyopathies
Bachelor mentorship
Csergeova Lucia
Genotyping of tumor biomarkers using novel multi-parallel sequencing technologies with an emphasis on ultra sesnsitive typing of circulating tumor DNA
Blaťák Ondřej
Novel approaches for diagnostics of mutations of genes involved in age-related clonal haematopoiesis
Open positions
1 Medical biology
| Project: | Genetic and epigenetic biomarkers of cancer diseases |
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| Supervisors: | Slavkovský Rastislav Ph.D., Džubák Petr M.D., Ph.D., Hajdúch Marián M.D., Ph.D. |
| Available: | 3 |
| Intended for: | Doctoral training |
| Summary: | 3 places in full-time study |
1 Biomarkers: Identification and validation
| Project: | Genetic and epigenetic biomarkers in cancer |
|---|---|
| Supervisors: | Drábek Jiří Ph.D., Slavkovský Rastislav Ph.D., Hajdúch Marián M.D., Ph.D. |
| Available: | 3 |
| Intended for: | Doctoral training |
| Summary: | Clonal hematopoiesis of indeterminate potential (CHIP) has recently been described as a common phenomenon associated with aging. It is characterized by the accumulation of somatic mutations in cells of the hematopoietic system. Although CHIP is manifested by the expansion of certain cell clones, this condition is not accompanied by any morphological features of hematological neoplasia. However, it has been shown that the incidence of clonal hematopoiesis correlates with increased overall mortality and the risk of developing malignant transformation of hematopoietic cells as well as cardiovascular disease, such as ischemic stroke. To what extent and by what mechanisms clonal hematopoiesis contributes to disease development remains a question of current research. The main aim of the project will be to pinpoint the principal cells carrying CHIP somatic mutations, and to study their role in development and maintenance of atherosclerotic plaques, especially of those involved in development of stroke. The comparison of the phenotype of CHIP positive and negative cells will be of special interest. The use of cellular models not only include different types of leucocytes but circulatory progenitor endothelial cells as well. The study will involve elderly subjects with the positive presence of CHIP (>65 years). Subject will be characterized based on the presence or absence of ischemic stroke and the presence or absence of carotid stenosis by our clinical collaborators. The presence of somatic variants in 38 selected genes associated with CHIP will be tested in subjects of interest within our research group. The project will use various techiques including FACS, MACS, cell cultures, DNA isolation from small amount of cells, a highly sensitive sequencing method for DNA genotyping allowing detection of variant with less than 1% allelic frequency, DNA/RNA sequencing library preparation, deep massively parallel sequencing of panel of genes using unique molecular barcodes/indices, RNAseq, bioinformatics and data analysis with possibilities of calculations using high performance computing cluster, data management and statistical evaluation. |
1 Molecular and Translational Medicine
| Project: | Genetic and epigenetic biomarkers in health and disease |
|---|---|
| Supervisors: | Slavkovský Rastislav Ph.D., Džubák Petr M.D., Ph.D., Hajdúch Marián M.D., Ph.D., Drábek Jiří Ph.D., Koudeláková Vladimíra Ph.D. |
| Available: | 5 |
| Intended for: | Doctoral training |