Přejít k hlavnímu obsahu

About us

EATRIS-CZ can offer an access to high throughput screening and high content analysis platform – one of the largest academic installations worldwide, providing screening and high-volume biology data on a broad diversity of assays and detection systems. The described HTS platform is industry strong, modular and flexible, and allows testing in BSL3 and BSL2+ environment, screening in combination with ionizing radiation (X-rays), mass spectrometry, high content analysis and others. The screening assays provide leads for downstream drug research and development.
The HTS/HCA screening platform is based on a state-of-art robotic system provided by HighResBiosolutions Ltd. The system consists of three robotic arms, automatic incubators, liquid handlers for microliter and nanoliter volumes, sealers, de-sealers, centrifuges and readers for fluorescence, luminescence, absorbance and ionizing radiation. Also integrated with the system are wide-field or spinning disc confocal microscopes (Operetta, Yokogawa CV7000) equipped with software tools for image analysis and data evaluation. A critical part of the robotic system is the automatized chemical library, containing more than 110 000 compounds.


Support of research/drug development projects

In the field of uHTS/HCA and chemical biology/genetics, EATRIS-CZ offers cooperation in collaborative and contractual R&D projects, including tests of biological activities of small molecules.

Available tests focus on

  • Cytotoxicity (non/cancer, resistant cell lines)
  • Primary human normal and diseased cells
  • Cell cycle analysis (fixed and live cells)
  • Phenotypic screens using HCA
  • Cytoskeleton modulation/integrity
  • DNA, RNA, protein synthesis
  • DNA damage/repair activity, screening in combination with X-ray radiation
  • Protein-protein and protein-nucleic acids interactions
  • G-protein coupled receptor activity (adenosine, cannabinoid and opioid receptors)
  • Intracellular calcium flux assay
  • cAMP production upon modulation of adenylyl cyclase activity by G-protein coupled receptors
  • Mapping the most significant signaling pathways (eg. Hedgehog, Wnt, p53, KRAS, RAGE) via phosphorylation and reporter
  • Assays
  • Screening of demethylating agents using an in-house developed cell line
  • Custom made biochemical tests
  • Antimicrobial activity in G+/G- strains including drug resistant bacteria, mycobacterium strains, yeasts, filamentous fungi,
  • viruses and parasites under BSL2 and BSL3 conditions
  • Cellular 3D culture models (cytotoxicity, reporter studies, etc.)

Other services

Development and validation of homogenous mix-and-measure assay (384-well and 1536-well format) in tight collaboration with the users for:

  • Absorbance
  • Luminescence
  • Fluorescence polarization
  • Fluorescence intensity
  • Fluorescence resonance energy transfer (FRET), ALPHA-Screen, LANCE
  • Time resolved Fluorescence (TRF)
  • High Content Screening
  • Identification of new molecular target or new assay approach
  • Validation of assay on provided by customer or commercial chemical libraries (Lopac1280, Prestwick, Enzo)
  • Support in analysis of HCS data
  • Custom scripts for image analysis on Columbus, Acapella, MathLab and ImageJ
  • Availability of broad spectrum of reporter cell lines
  • Hit and Lead profiling and preclinical development of selected drug candidates
Do tohoto pole může být nahráno neomezené množství souborů.
Limit 256 MB.
Povolené typy: gif, jpg, jpeg, png, bmp, eps, tif, pict, psd, txt, rtf, html, odf, pdf, doc, docx, ppt, pptx, xls, xlsx, xml, avi, mov, mp3, mp4, ogg, wav, bz2, dmg, gz, jar, rar, sit, svg, tar, zip.
I understand, that by submitting the project proposal submission form above, I accept that EATRIS-CZ, represented by the national node, The Institute of Molecular and Translational Medicine (IMTM), Faculty of Medicine and Dentistry, Palacký University in Olomouc, will process my personal data only for the purpose of replying to the project proposal and related information provided in the form.
I understand the information and data provided above are of non-confidential nature and can be processed by EATRIS-CZ for the purposes of internal project evaluation and partnering