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Slavkovský Rastislav Ph.D.

Journals

ŠPORIKOVÁ, Z., R. SLAVKOVSKÝ, L. TUČKOVÁ, O. KALITA, M. HOUDOVÁ MEGOVÁ, J. EHRMANN, M. HAJDÚCH, L. HRABALEK, M. VAVERKA
IDH1/2 Mutations in Patients With Diffuse Gliomas. Applied Immunohistochemistry and Molecular Morphology. 2021, 30(3), 178-183, ISSN: 1541-2016, PMID: 35262523,
SLAVKOVSKÝ, R., J. STRÁNSKÁ, V. VĚNSKOVÁ, S. JANČÍK, L. KOTKOVÁ, P. VOJTA, P. FLODR, M. BARTOUŠKOVÁ, H. ŠTUDENTOVÁ, M. HAJDÚCH, J. DRÁBEK
Development and extensive analytical validation of deep amplicon sequencing for detecting KRAS and NRAS mutations in metastatic colorectal cancer samples. Neoplasma. 2021, 69(1), 203-2015, ISSN: 0028-2685, PMID: 34881628,
KALITA, O., Z. ŠPORIKOVÁ, M. HAJDÚCH, M. HOUDOVÁ MEGOVÁ, R. SLAVKOVSKÝ, L. HRABALEK, M. HALAJ, Y. KLEMENTOVÁ, M. DOLEZAL, J. DRÁBEK, L. TUCKOVA, J. EHRMANN, J. VRBKOVÁ, R. TROJANEC, M. VAVERKA
The Influence of Gene Aberrations on Survival in Resected IDH Wildtype Glioblastoma Patients: A Single-Institution Study. Current Oncology. 2021, 28(2), 1280-1293, ISSN: 1198-0052, PMID: 33801093,  PDF.

Master mentorship

Podhorská Natália

Epigenetic changes detection in cancer cells

Stav: Absolvoval od 2015 do 2017.
Csergeova Lucia

Genotyping of tumor somatic biomarkers using multi-parallel sequencing technologies with molecular barcodes features

Stav: Absolvoval od 2019 do 2021.
Vysloužilová Lenka

Significance assessment of gene variants identified by next-generation sequencing associated with inherited cardiomyopathies

Stav: Absolvoval od 2017 do 2019.

Bachelor mentorship

Csergeova Lucia

Genotyping of tumor biomarkers using novel multi-parallel sequencing technologies with an emphasis on ultra sesnsitive typing of circulating tumor DNA

Stav: Absolvoval od 2017 do 2019.
Blaťák Ondřej

Novel approaches for diagnostics of mutations of genes involved in age-related clonal haematopoiesis

Stav: Probíhající od 2023.

Open positions

Projekt: Genetic and epigenetic biomarkers of cancer diseases
Vedoucí: Slavkovský Rastislav Ph.D., Džubák Petr M.D., Ph.D., Hajdúch Marián M.D., Ph.D.
K dispozici: 3
Určeno pro: Doktorské studium
Souhrn: 3 places in full-time study
Projekt: Genetic and epigenetic biomarkers in cancer
Vedoucí: Drábek Jiří Ph.D., Slavkovský Rastislav Ph.D., Hajdúch Marián M.D., Ph.D.
K dispozici: 3
Určeno pro: Doktorské studium
Souhrn: Clonal hematopoiesis of indeterminate potential (CHIP) has recently been described as a common phenomenon associated with aging. It is characterized by the accumulation of somatic mutations in cells of the hematopoietic system. Although CHIP is manifested by the expansion of certain cell clones, this condition is not accompanied by any morphological features of hematological neoplasia. However, it has been shown that the incidence of clonal hematopoiesis correlates with increased overall mortality and the risk of developing malignant transformation of hematopoietic cells as well as cardiovascular disease, such as ischemic stroke. To what extent and by what mechanisms clonal hematopoiesis contributes to disease development remains a question of current research. The main aim of the project will be to pinpoint the principal cells carrying CHIP somatic mutations, and to study their role in development and maintenance of atherosclerotic plaques, especially of those involved in development of stroke. The comparison of the phenotype of CHIP positive and negative cells will be of special interest. The use of cellular models not only include different types of leucocytes but circulatory progenitor endothelial cells as well. The study will involve elderly subjects with the positive presence of CHIP (>65 years). Subject will be characterized based on the presence or absence of ischemic stroke and the presence or absence of carotid stenosis by our clinical collaborators. The presence of somatic variants in 38 selected genes associated with CHIP will be tested in subjects of interest within our research group. The project will use various techiques including FACS, MACS, cell cultures, DNA isolation from small amount of cells, a highly sensitive sequencing method for DNA genotyping allowing detection of variant with less than 1% allelic frequency, DNA/RNA sequencing library preparation, deep massively parallel sequencing of panel of genes using unique molecular barcodes/indices, RNAseq, bioinformatics and data analysis with possibilities of calculations using high performance computing cluster, data management and statistical evaluation.
Projekt: Genetic and epigenetic biomarkers in health and disease
Vedoucí: Slavkovský Rastislav Ph.D., Džubák Petr M.D., Ph.D., Hajdúch Marián M.D., Ph.D., Drábek Jiří Ph.D., Koudeláková Vladimíra Ph.D.
K dispozici: 5
Určeno pro: Doktorské studium