Mistrík Martin Ph.D.
About me
Specializing in cellular stresses and their link to cancer development and treatment. Another specialization includes advanced microscopic techniques (the head of the bioimaging core facility: https://imtm.cz/core-facilities/bioimaging-core-facility ). Actively participated in the research of more than 10 national and international projects. Published more than >40 original investigations and three patents, >3000 citations, H-index 24
Journals
DNA damage-related ubiquitinations: one E1 that rules them all.
Cell Cycle.
2012,
11(10),
1872-1877,
ISSN: 1538-4101,
PMID: 22580454,
CDK targeting of NBS1 promotes DNA-end resection, replication restart and homologous recombination.
EMBO Reports.
2012,
13(6),
561-568,
ISSN: 1469-221X,
PMID: 22565321,
Thresholds of replication stress signaling in cancer development and treatment.
Nature Structural & Molecular Biology.
2012,
19(1),
5-7,
ISSN: 1545-9993,
PMID: 22218289,
Open positions
| Project: | DNA damage signaling in the cellular response to stress |
|---|---|
| Supervisors: | Mistrík Martin Ph.D. |
| Available: | 1 |
| Intended for: | Doctoral training |
| Summary: | 1 place in full-time study |
| Project: | Signaling DNA damage in cellular response to stress |
|---|---|
| Supervisors: | Mistrík Martin Ph.D. |
| Available: | 1 |
| Intended for: | Doctoral training |
| Summary: | Our DNA is constantly under threat from DNA damaging agents. If unrepaired DNA damage can lead to errors during genome duplication, including the mutations that can lead to cancer, contribute to aging and other human diseases. Cells have evolved elaborate repair mechanisms to fix this damage and ensure that the genetic information is faithfully reproduced, and we would like to understand these repair mechanisms at the molecular level. We ultimately aim for a complete molecular and cellular understanding of critical DNA repair pathways that act on DNA damage. The Ph.D. student will practice several molecular and cellular techniques, including cell culture, RNA interference, immunofluorescence microscopy, live-cell imaging and immunoprecipitation of proteins. He/she will also learn novel and powerful techniques, such as super-resolution microscopy and purification of proteins on newly synthesized DNA. |
| Project: | Study of DNA double-strand break repair in tumor model |
|---|---|
| Supervisors: | Mistrík Martin Ph.D. |
| Available: | 1 |
| Intended for: | Doctoral training |
| Summary: | The so-called double-strand breaks (DSB) are the most lethal DNA lesions, which can be potent sources of mutations and thus become the beginning of irreversible genetic changes leading to degenerative illnesses, including cancer. At the same induction of DSBs introduced in the proper setup can become an unsolvable problem for the cells, particularly in specific genetic backgrounds typical for cancer, which fact is the rationale for a plethora of cancer treatment strategies. Thus the way cells deal with DSBs is under the great interest of current medicinal and biological research. The Ph.D. student will practice several methods of induction of DSBs via various sources such as gamma rays, alpha particles, UV light and various chemical inducers. Next will be studied the molecular mechanisms the cell deal with this type of DNA damage. Emphasis will be put on differences between normal and cancer cells and their potential exploration for cancer therapy. Student will also learn mutiple laboratory techniques including cell culture, RNA/DNA transfections, advanced microscopic techniques, live-cell imaging, and various biochemical analysis of proteins. |
| Project: | Cellular stress in health and disease |
|---|---|
| Supervisors: | Mistrík Martin Ph.D., Moudrý Pavel Ph.D., Škrott Zdeněk Ph.D. |
| Available: | 3 |
| Intended for: | Doctoral training |
| Project: | DNA damage signaling in the cellular response to stress |
|---|---|
| Supervisors: | Mistrík Martin Ph.D. |
| Available: | 2 |
| Intended for: | Doctoral training |