Lišková Barbora Ph.D.
O mně
I am working in the pharmacology department with focus on the ADME. ADME (absorption, distribution, metabolism and excretion) deals with the study and prediction of the basic pharmacokinetic properties of drugs in their preclinical development. These in vitro models generatehe many ADME parameters, including chemical, plasma and microsomal stability and plasma protein binding. Another area of interest is permeability, for example, using the PAMPA method (Parallel artificial membrane permeability assay), which provides an in vitro model for passive diffusion. The models of cell lines (Caco-2, MDR1-MDCK) provide information such as permeability of substances across a cell monolayer. The MDR1-MDCK cell line is used to study P-glycoprotein substrates and inhibitors, as well as to assess the transfer of newly developed drugs across the blood-brain barrier. Caco-2, a cell line derived from colorectal cancer, is used to determine intestinal absorption. Analysis of samples is performed using a Agilent RapidFire 300 High-Throughput Mass Spectrometry System (Agilent, Wakefield, MA) with subsequent detection of the mass spectrometer Qtrap 5500 (AB Sciex, Concord, Canada), abbreviation RF-MS.
Journals
Open positions
| Projekt: | Pharmacokinetic methods in preclinical drug testing |
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| Vedoucí: | Hajdúch Marián M.D., Ph.D., Lišková Barbora Ph.D. |
| K dispozici: | 1 |
| Určeno pro: | Doktorské studium |
| Souhrn: | The study of ADME properties of a potential drug that are part of the IMTM chemical library - obtained by national or international cooperation belongs to one of the first steps in predicting potential drugs. In vitro models generate many ADME parameters, including chemical, plasma and microsomal stability, plasma protein binding and proportion of passive diffusion as a transport mechanism. The Caco-2 and MDCK-MDR1 permeability assays are established models of intestinal and blood-brain barriers, respectively. Analysis of samples is performed using a Agilent RapidFire 300 - rapid online solid phase extraction with subsequent detection of the mass spectrometer Qtrap 5500 (AB Sciex) - RF/MS. High-Throughput Mass Spectrometry System RF/MS delivers ultrafast, label – free analysis of native compounds for biochemical assays in ADME and provide throughput speeds of 6 to 10 seconds per sample. |
| Projekt: | Study of basic pharmacokinetic properties (ADME) of new drugs in preclinical development |
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| Vedoucí: | Lišková Barbora Ph.D. |
| K dispozici: | 1 |
| Určeno pro: | Doktorské studium |